Presence of ionotropic glutamate receptor subtypes in cultured rat retinal ganglion cells: a whole cell patch clamp study.
نویسندگان
چکیده
منابع مشابه
Whole-Cell Patch-Clamp Recordings
The patch-clamp recording technique, which measures ionic currents under voltage clamp, was designed to study small patches of membranes in which near perfect control of the transmembrane voltage can be achieved readily. The recent application of patch-clamp methodology to the analysis of whole-cell current actually defies many of the original design requirements. Nevertheless, whole-cell recor...
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Objective(s): The purpose of this study was to investigate the role of chloride channel protein 2 (ClC-2) in glutamate-induced apoptosis in the retinal ganglion cell line (RGC-5). Materials and Methods: RGC-5 cells were treated with 1 mM glutamate for 24 hr. The expression of ClC-2, Bax, and Bcl-2 was detected by western blot analysis. Cell survival and apoptosis were measured by 3-(4,5-dimeth...
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Human neurobalstoma cells, SH-SY5Y, are widely studied and data on their electrophysiological characteristics can be found in literature. We used this cell line to set up the whole cell patch-clamp technique, not yet available in our institute, with the goal to make it suitable both for our future scientific projects and for interdisciplinary studies with other research groups. Some instruments...
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ژورنال
عنوان ژورنال: Japanese Journal of Pharmacology
سال: 1999
ISSN: 0021-5198
DOI: 10.1016/s0021-5198(19)34584-6